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EGFR and HER2 expression in bladder cancer. ( A ) TCGA dataset was used to show increased expression of EGFR and HER2 in bladder cancer. (i) EGFR is significantly elevated in bladder cancer compared to normal urothelium ( P < 0.01; ANOVA), specifically in ‘basal’ subtype classes 3 and 4. (ii) HER2 is significantly elevated in bladder cancer compared to the normal urothelium ( P < 0.01; ANOVA), specifically in ‘luminal’ subtype classes 1 and 2. ( B ) Tissue microarrays <t>BL2081</t> and BL806 (Biomax) were analyzed for the presence of EGFR and HER2 expression. 232 BC samples were stained, and 186 (80%) samples showed presence of either EGFR, HER2, or both. Of those, 79 (42.4%) samples and 23 (12.4%) showed only EGFR expression and only HER2 expression, respectively. 84 (45.2%) samples showed co-expression of EGFR and HER2.
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A. Staining intensity of bladder cancer tissue cores by T stage. Tissue microarrays <t>BL2081</t> and BL806 (Biomax) were analyzed for presence of EGFR expression using anti-EGFR antibody (CST No. 4267). Each sample was graded for staining as follows: 0=<10% of cells positive (black); 1=10–24% (maroon); 2=25–49 % (red); 3=50–74% (firebrick); 4=75–100% (pink). There is no relationship between EGFR staining and T stage.
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EGFR and HER2 expression in bladder cancer. ( A ) TCGA dataset was used to show increased expression of EGFR and HER2 in bladder cancer. (i) EGFR is significantly elevated in bladder cancer compared to normal urothelium ( P < 0.01; ANOVA), specifically in ‘basal’ subtype classes 3 and 4. (ii) HER2 is significantly elevated in bladder cancer compared to the normal urothelium ( P < 0.01; ANOVA), specifically in ‘luminal’ subtype classes 1 and 2. ( B ) Tissue microarrays BL2081 and BL806 (Biomax) were analyzed for the presence of EGFR and HER2 expression. 232 BC samples were stained, and 186 (80%) samples showed presence of either EGFR, HER2, or both. Of those, 79 (42.4%) samples and 23 (12.4%) showed only EGFR expression and only HER2 expression, respectively. 84 (45.2%) samples showed co-expression of EGFR and HER2.

Journal: Scientific Reports

Article Title: Targeting Epidermal Growth Factor Receptor (EGFR) and Human Epidermal Growth Factor Receptor 2 (HER2) Expressing Bladder Cancer Using Combination Photoimmunotherapy (PIT)

doi: 10.1038/s41598-019-38575-x

Figure Lengend Snippet: EGFR and HER2 expression in bladder cancer. ( A ) TCGA dataset was used to show increased expression of EGFR and HER2 in bladder cancer. (i) EGFR is significantly elevated in bladder cancer compared to normal urothelium ( P < 0.01; ANOVA), specifically in ‘basal’ subtype classes 3 and 4. (ii) HER2 is significantly elevated in bladder cancer compared to the normal urothelium ( P < 0.01; ANOVA), specifically in ‘luminal’ subtype classes 1 and 2. ( B ) Tissue microarrays BL2081 and BL806 (Biomax) were analyzed for the presence of EGFR and HER2 expression. 232 BC samples were stained, and 186 (80%) samples showed presence of either EGFR, HER2, or both. Of those, 79 (42.4%) samples and 23 (12.4%) showed only EGFR expression and only HER2 expression, respectively. 84 (45.2%) samples showed co-expression of EGFR and HER2.

Article Snippet: Two commercially available TMAs were purchased from Biomax (Rockville, MD): BL2081 and BL806.

Techniques: Expressing, Staining

A. Staining intensity of bladder cancer tissue cores by T stage. Tissue microarrays BL2081 and BL806 (Biomax) were analyzed for presence of EGFR expression using anti-EGFR antibody (CST No. 4267). Each sample was graded for staining as follows: 0=<10% of cells positive (black); 1=10–24% (maroon); 2=25–49 % (red); 3=50–74% (firebrick); 4=75–100% (pink). There is no relationship between EGFR staining and T stage.

Journal: Molecular cancer therapeutics

Article Title: Epidermal Growth Factor Receptor (EGFR) Targeted Photoimmunotherapy (PIT) for the Treatment of EGFR expressing Bladder Cancer

doi: 10.1158/1535-7163.MCT-16-0924

Figure Lengend Snippet: A. Staining intensity of bladder cancer tissue cores by T stage. Tissue microarrays BL2081 and BL806 (Biomax) were analyzed for presence of EGFR expression using anti-EGFR antibody (CST No. 4267). Each sample was graded for staining as follows: 0=<10% of cells positive (black); 1=10–24% (maroon); 2=25–49 % (red); 3=50–74% (firebrick); 4=75–100% (pink). There is no relationship between EGFR staining and T stage.

Article Snippet: Tissue microarrays BL2081 and BL806 (Biomax) were analyzed for presence of EGFR expression using anti-EGFR antibody (CST No. 4267).

Techniques: Staining, Expressing

A. Staining intensity of bladder cancer tissue cores by T stage. Tissue microarrays BL2081 and BL806 (Biomax) were analyzed for presence of EGFR expression using anti-EGFR antibody (CST No. 4267). Each sample was graded for staining as follows: 0=<10% of cells positive (black); 1=10–24% (maroon); 2=25–49 % (red); 3=50–74% (firebrick); 4=75–100% (pink). There is no relationship between EGFR staining and T stage.

Journal: Molecular cancer therapeutics

Article Title: Epidermal Growth Factor Receptor (EGFR) Targeted Photoimmunotherapy (PIT) for the Treatment of EGFR expressing Bladder Cancer

doi: 10.1158/1535-7163.MCT-16-0924

Figure Lengend Snippet: A. Staining intensity of bladder cancer tissue cores by T stage. Tissue microarrays BL2081 and BL806 (Biomax) were analyzed for presence of EGFR expression using anti-EGFR antibody (CST No. 4267). Each sample was graded for staining as follows: 0=<10% of cells positive (black); 1=10–24% (maroon); 2=25–49 % (red); 3=50–74% (firebrick); 4=75–100% (pink). There is no relationship between EGFR staining and T stage.

Article Snippet: Commercially available tissue array blocks BL2081 and BL 806 (Biomax, Rockville, MD) were similarly stained with anti-EGFR antibody to detect the expression of EGFR in bladder tumors of various stages, grades, and histologies as well as adjacent normal bladder tissues.

Techniques: Staining, Expressing